Lukáš Vasilek studied conducting at the Academy of Performing Arts in Prague, and musicology at the Faculty of Arts, Charles University in Prague. For eleven seasons from 1998 he was conductor of the Foerster Female Chamber Choir, and between 2005 and 2007 simultaneously also second choirmaster of the Prague National Theatre opera chorus.

In 2007 he was appointed principal conductor of the Prague Philharmonic Choir. Apart from studying and conducting the choir´s own concert productions, he has been preparing the ensemble for participation in larger-scale cantata, oratorio and opera projects, working with leading international conductors (Barenboim, Bělohlávek, Eschenbach, Honeck, Hrůša, Jordan, Luisi, Mehta or Rattle, among others) and orchestras (including the Berliner Philharmoniker, Czech Philharmonic, Israel Philharmonic, Staatskapelle Dresden or Wiener Symphoniker). Since 2010 the PFS under his direction has guest appeared regularly at the Bregenz Festival, Austria. Lukáš Vasilek is signed under numerous recordings made by the Prague Philharmonic Choir for various major labels, including Decca, Deutsche Grammophon, Sony Classical and Supraphon. In 2016 the last mentioned of these released an album of Bohuslav Martinů´s cantatas which was recently singled out for special plaudits by the prestigious British magazines, Gramophone (Editor´s Choice), and BBC Music Magazine (Choral & Song Choice).

In 2010 Lukáš Vasilek formed the Martinů Voices chamber choir whose repertoire he has focused primarily on choral music encompassing the time span between the 19th and 21st centuries. He has taken the Martinů Voices to the Czech Republic´s major music festivals, as well as preparing the choir for work with leading Czech orchestras and conductors. Figuring prominently among his recordings with the Martinů Voices is an album of choral works by Jan Novák (Supraphon, 2014). Apart from pursuing his career as a choir conductor, Lukáš Vasilek devotes part of his time to occasional commitments as an orchestra conductor.